Performance Specification of the Laboratories

Performance Specification of the Laboratories for doping control
required by the International Federation of Horseracing Authorities

(cf. to Article 6 A § 18: http://www.ifhaonline.org/default.asp?section=IABRW&area=2#a6a)

 

This specification describes the minimum analytical capability required to detect exposure. It must not be construed as restricting in any way the chemical groups or concentrations that may constitute positive findings. Many laboratories will be able to surpass this specification, and the interpretation of whether a finding and a particular concentration would constitute a violation is governed by the relevant Rules of Racing.

Procedures should ensure that the laboratory can find prohibited substances in equine body fluids and prove their presence. Test methods must cover the chemical groups typified by the representative list. Each substance in the representative list must be detectable and identifiable at the concentration shown, if necessary after hydrolysis of metabolic conjugates. In addition, test methods must also reliably quantify threshold substances at concentrations that would be declared positive.

Representative list

Minimum concentations (ng/mL in horse urine) for detecting exposure

Amitriptyline

50 

Amphetamine

100

Atenolol

100

Benzoylecgonine

20

Boldenone

20

Bumetanide

10

Butorphanol

5

Caffeine / Caféïne

100

Chlorpromazine

20

Clenbuterol

4

Dexamethasone

2

Ephedrine

100

Ethacrynic acid / Acide éthacrynique

100

Flunixin / Flunixine

50

Hydrochlorothiazide

200

Mepivacaine

50

Methocarbamol

300

Morphine (analyte)

60 ng/mL of Morphine-3-glucuronide (in horse urine)
60 ng/mL de morphine-3-glucuronide (dans l’urine)

Nikethamide

100

Nordiazepam

100

Pethidine

20

Phenazone

300

Phenobarbitone

50

N.B. : These concentrations must not be construed as regulatory thresholds.