Performance Specification of the Laboratories

Performance Specification of the Laboratories for doping control
required by the International Federation of Horseracing Authorities

(cf. to Article 6 A § 19: http://www.ifhaonline.org/default.asp?section=IABRW&area=2#article6a)

 

This specification describes the minimum analytical capability required to detect exposure. It must not be construed as restricting in any way the chemical groups or concentrations that may constitute positive findings. Many laboratories will be able to surpass this specification, and the interpretation of whether a finding and a particular concentration would constitute a violation is governed by the relevant Rules of Racing.

Procedures should ensure that the laboratory can find prohibited substances in equine body fluids and prove their presence. Test methods must cover the chemical groups typified by the representative list. Each substance in the representative list must be detectable and identifiable at the concentration shown, if necessary after hydrolysis of metabolic conjugates. In addition, test methods must also reliably quantify threshold substances at concentrations that would be declared positive.

Note 1: These concentrations must not be construed as regulatory thresholds or screening limits.
Note 2: Additional and/or more stringent requirements applicable to the group of IFHA Reference Laboratories can be found in Annexure C of the IFHA Reference Laboratory White Manual, https://www.ifhaonline.org/default.asp?section=IABRW&area=13

 
Representative substance Minimum concentration (ng/mL in horse urine) for detecting exposure
2-(1-Hydroxyethyl)promazine sulfoxide 10
16β-Hydroxy-stanozolol 1     
Amitriptyline 50 
Amphetamine 10
Atenolol 10
Benzoylecgonine 20
Betamethasone 0.2
Boldenone or Boldenone sulfate (analyte) 40 ng/mL Boldenone sulfate (in horse urine) 
Bumetanide 10
Butorphanol 5
Caffeine 100
Chlorpromazine 20
Clenbuterol 2
Dermorphin 10
Detomidine, 3'-hydroxy 5
Dexamethasone 0.2
Ephedrine 20
Epitrenbolone (17α-Trenbolone) 1
Ethacrynic acid 100
Firocoxib 20
Fludrocortisone 10
Flufenamic acid 50
Flumethasone 20
Flunixin 50
Fluoxetine 20
Fluphenazine 5
Frusemide 100
Glycopyrrolate 2
Guanabenz 10
Hydrochlorothiazide 200
Hydromorphone 2
Ibuprofen 100
Ipratropium 1
Ketoprofen 100
Ketorolac 100
Lidocaine, 3'-hydroxy 25
Meclofenamic Acid 100
Meloxicam 25
Mephentermine 10
Mepivacaine, 3’-hydroxy 10
Methocarbamol 200
Morphine or Morphine-3-glucuronide (analyte) 50 ng/mL Morphine-3-glucuronide (in horse urine)
Nikethamide 100
Nordazepam 20
Oxazepam 20
Oxymorphone 2
Pethidine (Meperidine) 10
Phenazone (Antipyrine) 300
Phenobarbitone (Phenobarbital) 50
Pseudoephedrine 20
Ractopamine 5
Ritalinic acid 20
Salbutamol (Albuterol) 2
Sildenafil 5
Tenoxicam 20
Terbutaline 10
Tramadol, O-desmethyl 5
Triamcinolone acetonide 2
Venlafaxine, O-desmethyl 5
Xylazine, 4'-hydroxy 10
 
Representative substance Minimum concentration (ng/mL in horse plasma) for detecting exposure
Acepromazine 1
Amitriptyline 1
Atenolol 1
Benzoylecgonine 2
Betamethasone 0.2
Boldenone 0.1
Butorphanol 1    
Caffeine 10
Chlorpromazine 1
Clenbuterol 1
Codeine 10
Detomidine 0.1
Dexamethasone 0.2
Diazepam 10
Diclofenac 5
Ephedrine 10
Firocoxib 20
Flufenamic acid 10
Flumethasone 1
Flunixin 20
Fluphenazine 0.2
Ketamine 1
Ketoprofen 10
Lidocaine 1
Meloxicam 5
Mepivacaine 0.1
Methocarbamol 1
Methylphenidate 1
Methylprednisolone 0.1
Nandrolone 1
Nordazepam 1
Pethidine (Meperidine) 20
Pentazocine 1
Piroxicam 5
Pseudoephedrine 10
Prednisolone 5
Reserpine 1
Salbutamol (Albuterol) 1
Sildenafil 1
Stanozolol 1
Terbutaline 5
Testosterone 0.5
Tramadol 1
Triamcinolone acetonide 0.1
Xylazine 0.1